I cannot read it and agree with claims that peer review “is broken” and does not work. Such claims are at best oversimplifying the problems and selectively excluding evidence.
Why do a PhD?
Is doing a PhD worthwhile? Why do a PhD? These questions might appear simple, and yet they may have many answers depending on our goals and motivations. Here, I will share what I find worthwhile in pursuing a PhD, what motivated me almost 20 years ago to perform PhD research. I always thought of doctoral … Continue reading Why do a PhD?
Guidelines for single-cell proteomic experiments
Henry Ford remarked that coming together is a beginning, staying together is progress, and working together is success. Working together is also more fun. Thus, from the very beginning, we sought to help colleagues adopt and use single-cell proteomic methodologies that our laboratory developed, and to do so following best practices To develop and disseminate … Continue reading Guidelines for single-cell proteomic experiments
Adsorption losses during single-cell proteomics
Proteins are wonderfully diverse! Their physicochemical diversity is essential to animating life, to performing the myriad functions necessary to process energy, nutrients and information in living systems. The physicochemical diversity of proteins also poses challenges to proteomic analysis. Different amino acid sequences can adsorb to various surfaces, thus resulting in losses, reduced sensitivity and even … Continue reading Adsorption losses during single-cell proteomics
Framework for increasing proteomics throughput
Simultaneously achieving achieving deep proteome coverage, high data completeness, high throughput, and high sensitivity has been a long standing challenge in the field of proteomics. This challenge is particularly evident and relevant to single-cell proteomics, which has the potential to enable mechanistic investigations of key biological questions, such as signaling mechanisms based on protein binding, modifications, and … Continue reading Framework for increasing proteomics throughput
Let's promote good research: Share it in accessible and engaging ways. Put it in context and help your colleagues appreciate it. The more we can put substance ahead of hype, the more science and our colleagues benefit from our highlights.
Accessible single-cell proteomics
This aim for a “model T” single-cell proteomics has been our guiding philosophy in the development of methods for single-cell proteomics.
Label-free single-cell proteomics
Recently, Matthias Mann and colleagues published a preprint (doi: 10.1101/2020.12.22.423933v1) reporting a label-free mass-spectrometry method for single-cell proteomics. Many colleagues asked me what I think about the preprint, and I summarized a few comments in the peer review below. I did not examine all aspects of the work, but I hope my comments are useful: … Continue reading Label-free single-cell proteomics
My experience with elite journals
Over the last 15 years, my opinion about the significance of publishing in elite journals has evolved considerably. Below are some of main phases and the factors that have shaped my opinion: As a beginning PhD student, I took several classes based on discussing primary research papers. Many of these papers, especially in my biochemistry course, … Continue reading My experience with elite journals
Promoting (your) research
"I am a scientist focused on conducting research, not on promoting it." This thinking strongly resonated with me when I was a PhD student. If it resonates with you, read on to learn why and how you should promote (your) research. Common approaches to promoting your research include aiming to publish your papers in elite … Continue reading Promoting (your) research